The open source clustering software available
here implement the most commonly used clustering methods for
gene expression data analysis. The clustering methods can
be used in several ways. Cluster 3.0
provides a Graphical User Interface to access to the
clustering routines. It is available for Windows, Mac OS X,
and Linux/Unix. Python users can access the clustering
routines by using Pycluster,
which is an extension module to Python. People that want to
make use of the clustering algorithms in their own C, C++,
or Fortran programs can download the
source code of the C Clustering
Cluster 3.0 is an enhanced version of Cluster,
which was originally developed by
Michael Eisen while at
Cluster 3.0 was built for the Microsoft Windows platform,
and later ported to Mac OS X (Cocoa build for
Mac OS X v10.0 or later) and to Linux/Unix using Motif.
In addition to the GUI program, Cluster 3.0 can also be
run as a command line program.
For more information, please consult the
For Microsoft Windows and
Mac OS X, use the appropriate
installer. The Cluster 3.0 executables
cluster.com (on Windows) or
Cluster (on Mac OS X) can be used both
as a GUI program and as a command line program.
For Cluster 3.0 on Linux/Unix, you will need the
Motif libraries, which are already installed on many
Linux/Unix computers. You will need a version compliant
with Motif 2.1, such as
Cluster 3.0 can then be installed by typing
The resulting executable
cluster can be run as
a GUI program and as a command line program. For the latter,
you will need to use the appropriate
command line options.
If you are not interested in the GUI, and you want to run
Cluster 3.0 as a command line program only, you can install
a command-line only version of Cluster by typing
If you install Cluster 3.0 as a command-line only program
you do not need the Motif libraries.
Download (last update April 29, 2018; C Clustering Library version 1.56):
Installer for Microsoft Windows;
Installer for Mac OS X (Universal binary for PowerPC and Intel processors). You may need to remove /Library/Receipts/Cluster.pkg if you have an older version of Cluster 3.0 installed. If you get the error message '"Cluster.pkg" can't be opened because it is from an unidentified developer.', right-click on Cluster.pkg after downloading, and select "Open With" → "Installer".
Linux/Unix source code;
manual in PDF format.
To view the clustering results generated by Cluster 3.0, we
recommend using Alok Saldanha's
which can display hierarchical as well as k-means
clustering results. Java TreeView is not part of the Open
Source Clustering Software.
a scripting language with excellent support for numerical
work through the
package, providing a functionality similar to Matlab and R.
This makes Python together with Numerical Python an ideal
tool for analyzing genome-wide expression data.
Pycluster now uses the "new" Numerical Python
(version 1.3 or later).
Python can be easily integrated with C and other low-level
languages, thus combining the speed of C with the
flexibility of Python.
The routines available in Pycluster are described in the
manual to the C Clustering Library
. To install Pycluster, download the Pycluster source
distribution, unpack, change to the directory
Pycluster-1.56, and type
install as usual. If you use Python under Windows, we
recommend using the Windows installer instead, which is
available here for Python 2.7, 3.5, and 3.6.
Pycluster source distribution;
Windows installer for Python 2.7 (32 bits);
Windows installer for Python 2.7 (64 bits);
Windows installer for Python 3.5 (32 bits);
Windows installer for Python 3.5 (64 bits);
Windows installer for Python 3.6 (32 bits);
Windows installer for Python 3.6 (64 bits);
manual in PDF format.
Algorithm::Cluster, written by John Nolan of the
University of California,
Santa Cruz, is a Perl
module that makes use of the C Clustering Library. Some
example Perl scripts are available in the
perl/examples directory in the source
To install Algorithm::Cluster, download the source code,
unpack, and type
perl Makefile.PL, followed by
make to compile the code,
make test to run the test scripts, and
make install to install the Algorithm::Cluster
On Windows, we recommend using Strawberry Perl, which includes a compiler, allowing you to compile and install Algorithm::Cluster on Windows.
Download: Algorithm::Cluster source distribution;
manual in PDF format.
The routines in the C clustering library can be included in
or linked to other C programs (this is how we built
To use the C clustering library, simply collect the relevant
source files from the source code distribution.
As of version 1.04, the C clustering library complies
with the ANSI C standard.
manual in PDF format
The C clustering library and Pycluster were released under
the Python License. Algorithm::Cluster was released under
the Artistic License.
The GUI-codes Cluster 3.0 for Windows, Mac OS X, and
Linux/Unix, as well as the command line version of
Cluster 3.0 are still covered by the
original Cluster/TreeView license.
We would like to thank
Michael Eisen of
Berkeley Lab for making the
source code of Cluster/TreeView 2.11 available.
Without this source code, it would have been much harder to
develop Cluster 3.0.